Peanut (Arachis hypogaea) agglutinin (PNA)

Abstract

Peanut (Arachis hypogaea) agglutinin (PNA) is extensively used as tumour arker  it strongly recognises essentially the most cancers specific T antigen (Galbeta1–>3GalNAc-), nevertheless not its sialylated mannequin. Nonetheless, an additional specificity within the course of Galbeta1–>4GlcNAc (LacNAc), which is not tumour specific, had been attributed to PNA.

For correct interpretation of lectin histochemical outcomes we examined PNA sugar specificity using naturally occurring or semi-synthetic glycoproteins, matrix-immobilised galactosides and lectin-binding tissue glycoproteins, moderately than mono- or disaccharides as ligands. Dot-blots, swap blots or polystyrene plate coatings of the soluble glycoconjugates have been probed with horse-radish peroxidase (HRP) conjugates of PNA and totally different lectins of acknowledged specificity.

Modifications of PNA-binding glycoproteins, along with selective eradicating of O-linked oligosaccharides and remedy with glycosidases revealed that Galbeta1–>4GlcNAc (LacNAc) was ineffective whereas terminal alpha-linked galactose (TAG) along with uncovered T antigen (Galbeta1–>Three GalNAc-) was fantastic as sugar moiety in glycoproteins for his or her recognition by PNA. When immobilised, melibiose was superior to lactose in PNA binding. Outcomes have been confirmed using TAG-specific human serum anti-alpha-galactoside antibody.

Arachis hypogaea (Peanut) Lectin (PNA) – HRP

Affinity-purified PNA is conjugated to horseradish peroxidase (HRP) enzyme. HRP is usually used for blotting, immunoassays and immunohistochemistry methods. It is a 40 kDa protein that catalyzes the oxidation of substrates by hydrogen peroxide, resulting in a colored or fluorescent product or launch of sunshine as a byproduct of the response. This product is obtainable in a stabilized liquid kind.For substrate use of HRP-labelled protein detection we offer our Intensive ChemiLuminescence (ICLTM) tools (SKU: 20810000). ICL is a two-component substrate that accommodates a gentle luminol reply with an enhancer and a gentle peroxide buffer reply.

It has a robust light emission and a sensitivity at picogram diploma that is extraordinarily acceptable for the speedy detection of HRP-conjugated proteins. Advantages of chemiluminescent substrates over totally different detection methods incorporates a lot of exposures, blots is also reprobed, detects and quantitates a wide range of protein concentrations, and yields largest sensitivity of any on the market method.

arachis eylabs
arachis eylabs

We moreover provide 3, 3, 5, 5-Tetramethyl benzidine (TMB, SKU: 42000012) and TMB OneTM (TMB 1) HRP substrate for HRP labelled detection. TMB is an acceptable substrate for the immunoassay of horseradish peroxidase and is a noncarcinogenic by-product of benzidine with an absorbance at 450 nm. Oxidation of TMB by HRP yields a colored product. s TMB 1TM is a one ingredient peroxidase substrate (SKU: 21530073).

Biochem/Physiol Actions

PNA would not agglutinate common human erythrocytes, nevertheless strongly agglutinates neuraminidase dealt with erythrocytes. PNA has potent anti-T train very similar to the anti-T antibody in human sera. The lectin will be utilized to inform aside between human lymphocyte subsets.

Software program

Frequent Western Blot Protocol:

  • Glycoprotein sample dimension: 500ng
  • Lectin Focus: 0.1ug/ml
  1. Load samples at 500 ng of glycoprotein per lane
  2. Run 4-20% Bis-Tris SDS net web page gel
  3. Change gel to a PVDF membrane
  4. Block membrane for 1 hr at RT with RIPA buffer (R0278 Sigma)
  5. Incubate HRP lectin at 0.1ug/ml with RIPA buffer for 2 hours at RT
  6. Wash membrane 5 x 5 minutes with 25ml RIPA buffer
  7. Detect using chemiluminescent substrate (CPS1-120)

Unit Definition

One unit will kind 1 mg purpurogallin in 20 sec from pyrogallol at pH 6.Zero at 20 °C.

Bodily kind

Accommodates sodium citrate

Preparation Discover

Prepared from peroxidase (P8375) using a modification of a broadcast method, which favors low molecular weight conjugates. Repurified after conjugation by affinity chromatography.

Arachis hypogaea Conglutin-7

1-CSB-EP757666ANE
  • EUR 611.00
  • EUR 309.00
  • EUR 1827.00
  • EUR 939.00
  • EUR 1218.00
  • EUR 397.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Arachis hypogaea Conglutin-7 expressed in E.coli

Arachis hypogaea Arachin Ahy-3

1-CSB-EP737391ANE
  • EUR 611.00
  • EUR 309.00
  • EUR 1827.00
  • EUR 939.00
  • EUR 1218.00
  • EUR 397.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Arachis hypogaea Arachin Ahy-3 expressed in E.coli

Arachis hypogaea Arachin Ahy-3

1-CSB-YP737391ANE
  • EUR 679.00
  • EUR 335.00
  • EUR 2172.00
  • EUR 1051.00
  • EUR 1442.00
  • EUR 435.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Arachis hypogaea Arachin Ahy-3,partial expressed in Yeast

Arachis hypogaea Allergen Ara h 1, clone P17

1-CSB-EP331779ANE(A4)
  • EUR 611.00
  • EUR 309.00
  • EUR 1827.00
  • EUR 939.00
  • EUR 1218.00
  • EUR 397.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Arachis hypogaea Allergen Ara h 1, clone P17 expressed in E.coli

Arachis hypogaea Allergen Ara h 1, clone P17

1-CSB-EP331779ANE
  • EUR 611.00
  • EUR 309.00
  • EUR 1827.00
  • EUR 939.00
  • EUR 1218.00
  • EUR 397.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Arachis hypogaea Allergen Ara h 1, clone P17,partial expressed in E.coli

Arachis hypogaea Allergen Ara h 1, clone P17

1-CSB-YP331779ANE(A4)
  • EUR 586.00
  • EUR 299.00
  • EUR 2172.00
  • EUR 900.00
  • EUR 1442.00
  • EUR 382.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Arachis hypogaea Allergen Ara h 1, clone P17 expressed in Yeast

Arachis hypogaea Allergen Ara h 1, clone P41B

1-CSB-CF337356ANE
  • EUR 965.00
  • EUR 665.00
  • EUR 715.00
  • 1MG
  • 200ug
  • 500ug
Description: Recombinant Arachis hypogaea Allergen Ara h 1, clone P41B expressed in in vitro E.coli expression system

Biotin [HRP]

DAG3197-HRP 1mg
EUR 429

Vitamine B9 [HRP]

DAGDT1440-HRP 1mg
EUR 1105

Vitamine B12 [HRP]

DAGT5413-HRP 1mg
EUR 975

GAPDH Antibody - HRP

T0004-HRP 1ml
EUR 920
Description: GAPDH Mouse Monoclonal antibody detects endogenous levels of total GAPDH protein.

CF488A Arachis hypogaes Lectin PNA (Peanut)

29060 1mg
EUR 195
Description: Minimum order quantity: 1 unit of 1mg

CF568 Arachis hypogaes Lectin PNA (Peanut)

29061 1mg
EUR 195
Description: Minimum order quantity: 1 unit of 1mg

CF594 Arachis hypogaes Lectin PNA (Peanut)

29062 1mg
EUR 195
Description: Minimum order quantity: 1 unit of 1mg

CF640R Arachis hypogaes Lectin PNA (Peanut)

29063 1mg
EUR 195
Description: Minimum order quantity: 1 unit of 1mg

HRP-IgG Conjugation Kit

55R-HRP 1 mg
EUR 705
Description: HRP conjugation kit for all types of IgG

beta-Actin Antibody - HRP

T0022-HRP 1ml
EUR 920
Description: beta-Actin Mouse Monoclonal antibody detects endogenous levels of total beta-Actin protein.

Goat Anti-Rat IgG1-HRP Conjugate

50121-HRP 0.5 ml
EUR 225

Goat Anti-Rat IgG2a-HRP Conjugate

50122-HRP 0.5 ml
EUR 225

Goat Anti-Rat IgG2b-HRP Conjugate

50123-HRP 0.5 ml
EUR 225

Goat Anti-Rat IgG2c-HRP Conjugate

50124-HRP 0.5 ml
EUR 225

Monoclonal Anti-Rat IgG1-HRP Conjugate

50126-HRP 0.5 ml
EUR 286

Rabbit Anti-Bird IgM-HRP Conjugate

90521-HRP 100 ul
EUR 347

Concanavalin A Lectin (ConA)-HRP conjugate

CONA15-HRP 0.5 ml
EUR 286

Aleuria Aurantia Lectin (Aal)-HRP conjugate

AAL15-HRP 0.5 ml
EUR 225

Monoclonal Anti-Poly His-HRP conjugate

HISP12-HRP 100 ul
EUR 347

Lens culinaris Lectin (LCA)-HRP conjugate

LCA15-HRP 0.5 ml
EUR 286

Sambucus nigra Lectin (SNA)-HRP conjugate

SNA15-HRP 0.5 ml
EUR 286

Anti-Per.C6Ð’ Affinity Purified HRP, Goat

PC118AF-HRP 1 mg
EUR 4069
Description: Anti-Per.C6Ð’ Affinity Purified HRP, Goat by Cygnus Technologies is available in Europe via Gentaur.

Rabbit Anti-Salmonella IgG-HRP conjugate

SLM11-HRP 0.5 ml
EUR 347

Goat Anti-Donkey IgG (Fc)-HRP conjugate

80950-HRP 0.5 ml
EUR 225

Rabbit Anti-bovine Catalase IgG-HRP Conjugate

CATB11-HRP 100 ul
EUR 469

Sheep Anti-rat albumin IgG-HRP Conjugate

ALBR12-HRP 100 ul
EUR 347

Goat Anti-human lactoferrin IgG-HRP Conjugate

LTF11-HRP 0.5 ml
EUR 445

Goat Anti-bovine lactoferrin IgG-HRP conjugate

LTF13-HRP 0.5 ml
EUR 445

Goat Anti-Human Transferrin IgG-HRP conjugate

TF11-HRP 100 ug
EUR 347

Goat Anti-Mouse Transferrin IgG-HRP conjugate

TF14-HRP 100 ug
EUR 347

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