Peanut (Arachis hypogaea) agglutinin (PNA) is extensively used as tumour arker it strongly recognises essentially the most cancers specific T antigen (Galbeta1–>3GalNAc-), nevertheless not its sialylated mannequin. Nonetheless, an additional specificity within the course of Galbeta1–>4GlcNAc (LacNAc), which is not tumour specific, had been attributed to PNA.
For correct interpretation of lectin histochemical outcomes we examined PNA sugar specificity using naturally occurring or semi-synthetic glycoproteins, matrix-immobilised galactosides and lectin-binding tissue glycoproteins, moderately than mono- or disaccharides as ligands. Dot-blots, swap blots or polystyrene plate coatings of the soluble glycoconjugates have been probed with horse-radish peroxidase (HRP) conjugates of PNA and totally different lectins of acknowledged specificity.
Modifications of PNA-binding glycoproteins, along with selective eradicating of O-linked oligosaccharides and remedy with glycosidases revealed that Galbeta1–>4GlcNAc (LacNAc) was ineffective whereas terminal alpha-linked galactose (TAG) along with uncovered T antigen (Galbeta1–>Three GalNAc-) was fantastic as sugar moiety in glycoproteins for his or her recognition by PNA. When immobilised, melibiose was superior to lactose in PNA binding. Outcomes have been confirmed using TAG-specific human serum anti-alpha-galactoside antibody.
Arachis hypogaea (Peanut) Lectin (PNA) – HRP
Affinity-purified PNA is conjugated to horseradish peroxidase (HRP) enzyme. HRP is usually used for blotting, immunoassays and immunohistochemistry methods. It is a 40 kDa protein that catalyzes the oxidation of substrates by hydrogen peroxide, resulting in a colored or fluorescent product or launch of sunshine as a byproduct of the response. This product is obtainable in a stabilized liquid kind.For substrate use of HRP-labelled protein detection we offer our Intensive ChemiLuminescence (ICLTM) tools (SKU: 20810000). ICL is a two-component substrate that accommodates a gentle luminol reply with an enhancer and a gentle peroxide buffer reply.
It has a robust light emission and a sensitivity at picogram diploma that is extraordinarily acceptable for the speedy detection of HRP-conjugated proteins. Advantages of chemiluminescent substrates over totally different detection methods incorporates a lot of exposures, blots is also reprobed, detects and quantitates a wide range of protein concentrations, and yields largest sensitivity of any on the market method.
arachis eylabs
We moreover provide 3, 3, 5, 5-Tetramethyl benzidine (TMB, SKU: 42000012) and TMB OneTM (TMB 1) HRP substrate for HRP labelled detection. TMB is an acceptable substrate for the immunoassay of horseradish peroxidase and is a noncarcinogenic by-product of benzidine with an absorbance at 450 nm. Oxidation of TMB by HRP yields a colored product. s TMB 1TM is a one ingredient peroxidase substrate (SKU: 21530073).
Biochem/Physiol Actions
PNA would not agglutinate common human erythrocytes, nevertheless strongly agglutinates neuraminidase dealt with erythrocytes. PNA has potent anti-T train very similar to the anti-T antibody in human sera. The lectin will be utilized to inform aside between human lymphocyte subsets.
Software program
Frequent Western Blot Protocol:
Glycoprotein sample dimension: 500ng
Lectin Focus: 0.1ug/ml
Load samples at 500 ng of glycoprotein per lane
Run 4-20% Bis-Tris SDS net web page gel
Change gel to a PVDF membrane
Block membrane for 1 hr at RT with RIPA buffer (R0278 Sigma)
Incubate HRP lectin at 0.1ug/ml with RIPA buffer for 2 hours at RT
Wash membrane 5 x 5 minutes with 25ml RIPA buffer
Detect using chemiluminescent substrate (CPS1-120)
Unit Definition
One unit will kind 1 mg purpurogallin in 20 sec from pyrogallol at pH 6.Zero at 20 °C.
Bodily kind
Accommodates sodium citrate
Preparation Discover
Prepared from peroxidase (P8375) using a modification of a broadcast method, which favors low molecular weight conjugates. Repurified after conjugation by affinity chromatography.
Description: FITC Conjugated Arachis hypogaea Lectin (Peanut) -PNA-, 5mg Product volume may vary.
With the exception of Diagnostic Microbiology Products all biochemicals described are for research use only. The biochemical reagents are not designed for use in therapeutic or diagnostic applications. We are unable to ship to individuals. Please place all orders through an established firm or institution. Nothing disclosed is to be construed as a recommendation to use our products in violation of any patents. All research products are offered without warranty or guarantee, since the ultimate condition of use and the variability in material handling are beyond our control.
Description: TRITC Conjugated Arachis hypogaea Lectin (Peanut) -PNA-, 2mg Product volume may vary.
With the exception of Diagnostic Microbiology Products all biochemicals described are for research use only. The biochemical reagents are not designed for use in therapeutic or diagnostic applications. We are unable to ship to individuals. Please place all orders through an established firm or institution. Nothing disclosed is to be construed as a recommendation to use our products in violation of any patents. All research products are offered without warranty or guarantee, since the ultimate condition of use and the variability in material handling are beyond our control.
Description: Biotin Conjugated Arachis hypogaea Lectin (Peanut) -PNA-, 1mg Product volume may vary.
With the exception of Diagnostic Microbiology Products all biochemicals described are for research use only. The biochemical reagents are not designed for use in therapeutic or diagnostic applications. We are unable to ship to individuals. Please place all orders through an established firm or institution. Nothing disclosed is to be construed as a recommendation to use our products in violation of any patents. All research products are offered without warranty or guarantee, since the ultimate condition of use and the variability in material handling are beyond our control.
Description: Biotin Conjugated Arachis hypogaea Lectin (Peanut) -PNA-, 2mg Product volume may vary.
With the exception of Diagnostic Microbiology Products all biochemicals described are for research use only. The biochemical reagents are not designed for use in therapeutic or diagnostic applications. We are unable to ship to individuals. Please place all orders through an established firm or institution. Nothing disclosed is to be construed as a recommendation to use our products in violation of any patents. All research products are offered without warranty or guarantee, since the ultimate condition of use and the variability in material handling are beyond our control.
Description: Biotin Conjugated Arachis hypogaea Lectin (Peanut) -PNA-, 5mg Product volume may vary.
With the exception of Diagnostic Microbiology Products all biochemicals described are for research use only. The biochemical reagents are not designed for use in therapeutic or diagnostic applications. We are unable to ship to individuals. Please place all orders through an established firm or institution. Nothing disclosed is to be construed as a recommendation to use our products in violation of any patents. All research products are offered without warranty or guarantee, since the ultimate condition of use and the variability in material handling are beyond our control.
Description: Ferritin Conjugated Arachis hypogaea Lectin (Peanut) -PNA-, 1mg Product volume may vary.
With the exception of Diagnostic Microbiology Products all biochemicals described are for research use only. The biochemical reagents are not designed for use in therapeutic or diagnostic applications. We are unable to ship to individuals. Please place all orders through an established firm or institution. Nothing disclosed is to be construed as a recommendation to use our products in violation of any patents. All research products are offered without warranty or guarantee, since the ultimate condition of use and the variability in material handling are beyond our control.
Texas Red Conjugated Arachis hypogaea Lectin (Peanut) -PNA-
Description: Texas Red Conjugated Arachis hypogaea Lectin (Peanut) -PNA-, 2mg Product volume may vary.
With the exception of Diagnostic Microbiology Products all biochemicals described are for research use only. The biochemical reagents are not designed for use in therapeutic or diagnostic applications. We are unable to ship to individuals. Please place all orders through an established firm or institution. Nothing disclosed is to be construed as a recommendation to use our products in violation of any patents. All research products are offered without warranty or guarantee, since the ultimate condition of use and the variability in material handling are beyond our control.
Description: Alkaline Phosphatase Conjugated Arachis hypogaea Lectin (Peanut) -PNA-, 1mg With the exception of Diagnostic Microbiology Products all biochemicals described are for research use only. The biochemical reagents are not designed for use in therapeutic or diagnostic applications. We are unable to ship to individuals. Please place all orders through an established firm or institution. Nothing disclosed is to be construed as a recommendation to use our products in violation of any patents. All research products are offered without warranty or guarantee, since the ultimate condition of use and the variability in material handling are beyond our control.
Description: Colloidal Gold Conjugated Arachis hypogaea Lectin (Peanut) -PNA-, 5nm With the exception of Diagnostic Microbiology Products all biochemicals described are for research use only. The biochemical reagents are not designed for use in therapeutic or diagnostic applications. We are unable to ship to individuals. Please place all orders through an established firm or institution. Nothing disclosed is to be construed as a recommendation to use our products in violation of any patents. All research products are offered without warranty or guarantee, since the ultimate condition of use and the variability in material handling are beyond our control.
Description: Colloidal Gold Conjugated Arachis hypogaea Lectin (Peanut) -PNA-, 10nm With the exception of Diagnostic Microbiology Products all biochemicals described are for research use only. The biochemical reagents are not designed for use in therapeutic or diagnostic applications. We are unable to ship to individuals. Please place all orders through an established firm or institution. Nothing disclosed is to be construed as a recommendation to use our products in violation of any patents. All research products are offered without warranty or guarantee, since the ultimate condition of use and the variability in material handling are beyond our control.
Description: Colloidal Gold Conjugated Arachis hypogaea Lectin (Peanut) -PNA-, 15nm With the exception of Diagnostic Microbiology Products all biochemicals described are for research use only. The biochemical reagents are not designed for use in therapeutic or diagnostic applications. We are unable to ship to individuals. Please place all orders through an established firm or institution. Nothing disclosed is to be construed as a recommendation to use our products in violation of any patents. All research products are offered without warranty or guarantee, since the ultimate condition of use and the variability in material handling are beyond our control.
Description: Colloidal Gold Conjugated Arachis hypogaea Lectin (Peanut) -PNA-, 20nm With the exception of Diagnostic Microbiology Products all biochemicals described are for research use only. The biochemical reagents are not designed for use in therapeutic or diagnostic applications. We are unable to ship to individuals. Please place all orders through an established firm or institution. Nothing disclosed is to be construed as a recommendation to use our products in violation of any patents. All research products are offered without warranty or guarantee, since the ultimate condition of use and the variability in material handling are beyond our control.
Description: Colloidal Gold Conjugated Arachis hypogaea Lectin (Peanut) -PNA-, 30nm With the exception of Diagnostic Microbiology Products all biochemicals described are for research use only. The biochemical reagents are not designed for use in therapeutic or diagnostic applications. We are unable to ship to individuals. Please place all orders through an established firm or institution. Nothing disclosed is to be construed as a recommendation to use our products in violation of any patents. All research products are offered without warranty or guarantee, since the ultimate condition of use and the variability in material handling are beyond our control.
Description: Colloidal Gold Conjugated Arachis hypogaea Lectin (Peanut) -PNA-, 40nm With the exception of Diagnostic Microbiology Products all biochemicals described are for research use only. The biochemical reagents are not designed for use in therapeutic or diagnostic applications. We are unable to ship to individuals. Please place all orders through an established firm or institution. Nothing disclosed is to be construed as a recommendation to use our products in violation of any patents. All research products are offered without warranty or guarantee, since the ultimate condition of use and the variability in material handling are beyond our control.
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